Paul R. Selvin
University of Illinois at Urbana-Champaign
Department of Physics
1110 West Green Street
Urbana, IL 61801-3080
selvin@uiuc.edu
phone: (217)-244-3371 - fax: 244-7559
Home page:  http://people.physics.uiuc.edu/Selvin/PRS/PRS.html

Research Aims
To study the structure and dynamics of biological macromolecules using fluorescence. Specific biological systems of interest include molecular motors (conventional and unconventional myosins, kinesins, dyneins), voltage-controlled ion-channels (Shaker potassium), and more recently, genomics. The lab is split between technology development and biological applications. Technique development includes development of single molecule fluorescence, particularly modifications of Fluorescence Imaging with One Nanometer Accuracy (FIONA). These include DOPI (Defocused Orientation and Position Imaging of myosin V), SHREC (Single molecule High REsolution with Colocalization, also known as 2D-FIONA), SHRIMP (Super High Resolution IMaging with Photobleaching), and single-molecule fluorescence resonance energy transfer.

Research Accomplishments

• 1.5 nm spatial localization of single fluorescent molecules with 1-500 msec temporal resolution via Fluorescence Imaging with One Nanometer Accuracy (FIONA).
  
• Determination of hand-over-hand vs. inchworm model for single-molecule myosin V [Yildiz, Science,2003], myosin VI [Yildiz, J. Bio. Chem., 2004; Park, Mol. Cell, 2006], and kinesin [Yildiz, Science, 2004].
• Determination of step-size and rate of stepping of kinesin, dynein in vivo [Kural, Science, 2005].
  
• < 8 nm spatial resolution between two identical fluorescent dyes [Gordon, PNAS, 2004].
• < 8 nm spatial resolution between two differently-colored fluorescent dyes [Yildiz, Biophy. J., 2006].
• Development of Lanthanide Resonance Energy Transfer (LRET). This included a 100-fold improvement in signal to background over conventional fluorescence resonance energy transfer [Selvin, PNAS, 1994; Selvin, JACS, 1994].
• First detection of atomic-scale conformational changes in a voltage-controlled ion channel, using LRET [Cha, Nature, 1999].
• The S4 voltage-sensor does not undergo a large, voltage-dependent, membrane crossing: measured by LRET [Posson, Nature, 2006].
• First direct detection of an actin-dependent conformational change in myosin [Xiao, PNAS, 1998].
• An actin-dependent conformational change in myosin [Xiao, Nat. Struct. Bio., 2003].
• Detected single fluorescent molecules, their polarization states [Ha, PRL, 1996], quantum jumps [Ha, Chem. Phys. Let., 1997] spectra, and energy transfer between a single donor-acceptor pair via confocal and Near-Field Scanning Optical Microscopy [Ha, PNAS, 1996].
• Developed new luminescent lanthanide chelates that are alternatives to fluorescent dyes and are particularly useful for discriminating against background fluorescence. This includes their synthesis [Li, JACS, 1995], polarization [Reifenberger, J. Phys. Chem., 2003], and atomic (x-ray) structure [Purdy, Acta Cryst. 2002; Selvin, Inorganic Chem., 1996].
• Developed new class of luminescent dyes with tunable emission color and lifetime [Chen, JACS, 2000].
• Used picosecond fluorescence spectroscopy to detect anharmonic torsional modes in DNA [Selvin, Science, 1992].
  
  

Education

• Ph.D.: Physics, University of California-Berkeley (December 1990).
  • Thesis Title: "DNA Dynamics: From Picoseconds to Milliseconds." 
  • Research Director: Dr. Melvin Klein, Staff Scientist, Lawrence Berkeley Laboratory.
  • My research involved developing time-resolved Fluorescence and Electron Paramagnetic Resonance Spectrometers and applying them to study the structure and dynamics of well characterized DNA, both in vivo and in vitro.
• B.S.: Physics (Minor in Chemistry), University of Michigan, Ann Arbor, 1983. (3.93 GPA).

Professional Experience

Research

• Faculty Affiliate of Cell & Developmental Biology (2005-present)
• Professor, Departments of Physics and Biophysics, University of Illinois at Urbana-Champaign (Aug 04-present).
• Associate Professor, Departments of Physics and Biophysics, University of Illinois at Urbana-Champaign (Aug 00-Aug 04).
• Assistant Professor, Departments of Physics and Biophysics, University of Illinois at Urbana-Champaign (Aug 97-Aug 00).
• Staff Scientist, Life Science Division, Lawrence Berkeley Laboratory (Oct 95-July 97).
• Research Chemist, University of California-Berkeley, Chemistry Department (Nov. 93-Sept 95).
• Post-Doctoral Research Associate with Dr. John Hearst, University of California-Berkeley, Chemistry Department (1991-93).
• Graduate Student under Dr. Melvin Klein, University of California-Berkeley, Physics Department (1986-90).
• Undergraduate Research Assistant for Professors Dan Axelrod and John Langmore, University of Michigan (1980-83).
• Scientist (instrument development), Coulter Biomedical Research Corporation (Summers 1980-82).

Teaching

• Professor, "Single Molecule Biophysics (Grad seminar)," Physics 598IBT, UIUC, Fall 2007.
• Professor, "Introduction to Biological Physics Research (Undergrad seminar)," Physics 498IBT, UIUC, Fall 2006.
• Professor, "Thermal & Quantum Physics," Physics 213/214, UIUC, Fall 2005.
• Professor, "Introduction to Biological Physics," Physics 398Bio, UIUC, Spring 2003; Physics 498Bio, UIUC, Spring 2007.
• Developed and taught new course, "Introduction to Biological Physics," Physics 398Bio, UIUC, Spring 2002.
• Professor, "How Things Work," Phys140, UIUC, Fall 1998,  Spring 1999, Fall 1999, Fall 2000, Spring 2001.
• Thesis Committee, Scuola Normale Superiore, Pisa Italy, August 2001
• Professor, Laboratory for Introductory Physics (Mechanics, without Calculus), University of Illinois at Urbana-Champaign, Spring 1998, Fall 2002.
• Advisor to over a dozen graduate students, a dozen post-docs, and two undergraduates, 1997-present.
• Lecturer: Graduate-level Biophysical Chemistry, University of California-Berkeley, 1/3 semester, 1993.
• Teaching Assistant-Physics Dept., University of California-Berkeley, 4 semesters, 1983-86.
• Resource Person for High School Science Students, Lawrence Hall of Science (1989).
• Senior Thesis advisor to Sharad Shanbhag, 1991; Monique Farantzos, 1995.

Committees and Service

    National

• Conference co-organizer, American Chemical Society, Single Molecule, Boston, MA August 19-24, 2007.
• JGP Editorial Advisory Board, January 1, 2007
• American Chemical Society: co-organizer of Single Molecule Session, Aug. 2007.
• NIH Biophysics MFSC Study Section: June 2006
• Awards Committee, Biophysical Soc, 2005-2008
• Biophysical Society Council, 2004.
• Editorial Board, Bioconjugate Chemistry, 2004-
• Member, 2004 Program Committee for the Biophysics Society Annual Meeting.
• NIH BBCB Study Section Ad-Hoc Panel Member, February 2001. October 2001.
• NIH Advisory Committee on Single Molecule Detection, April 2000.
• NIH SBIR Study Section Ad-Hoc Reviewer, 1999, July 2001.
• Member, Editorial Board, Journal of Fluorescence, 1998-2001.
• NIH Study Section for R01's: channels: Nov. 2005.
• Reviewer for Journal of Neuroscience; Science; Proceedings of the National Acad. of Science; Biophysical Journal; Journal of Molecular Biology; Bioconjugate Chemistry; Analytical Biochemistry; Journal of Cell Biology; Trends in Cell Biology; Nature Structural & Molecular Biology; Journal of Fluorescence; Physical Biology; Journal of the American Chemical Society; BioMed Central, Nature Methods, and Nature Naontechnology.

    International

• Advisor to International Science and Technology Center (ISTC) #1734, via U.S. State Dept., "Development of an original, high-sensitive,express method for biological objects identification and control in water environment using scanning probe microscopy," Moscow, Russia. May 2001 - present.
• Thesis Committee, Scuola Normale Superiore, Pisa, Italy, Fall 2000.

    University

• Physics Appointments and Promotions Committee for AY 2005-.
  
• Executive Committee of the Molecular Biophysics Training Grant, 2003.
  
• Executive Committee of the Center for Biophysics and Computational Biology, 2003.
• Conference organizer, 50th Midwest Solid State Physics Conference, Univ. of Illinois, Urbana, Oct 18-20, 2002.
• Colloquim Chair, Physics Department, Fall 2001.
• Qualifying Examination Committee, Biophysics Center, Spring 2001.
• Qualifying Examination Committee, Physics Department, Fall 1999, Spring 2001.
• Undergraduate Curriculum Committee, Physics Department, Fall 2001.
• Panel member: "On being a successful academic: A guide for the perplexed," UIUC Office of Provost, May 24, 2001.
• Engineering-Chemistry Liason Subcommittee of Engineering College Executive Committee, 2000-02 (Chair, 2000-01).
• Physics/Molecular Biophysics Search Committee, Fall 1999-Spring 2000, Fall 2002.
• Guest lecturer: Physics 398 "Biophysics as a Career"  Fall 2000.
• Guest Lecturer, Physics 199B, "What a physicist can do for biology," Fall 2000.
• Biological Physics Curriculum Committee, Fall 2000.
• UIUC Research Board Proposals Reviewer, Fall 2000, Fall 2001
• Physics Advisory Committee, Fall 2000--Fall 2002.
• Molecular Biophysics Seminar Organizer, Fall 2000.
• Panel Member, General Engineering 393, "Business Opportunities in Biotech," Sept. 2000.
• Prelimary Examination Committee (approximately ten physics and biophysics students), Fall 1999-present.
• Thesis Committee (approximately twenty-five physics and biophysics students), Fall 1999-present.
• Undergraduate Physics Majors Advisor, Fall 1999-present.

Science Writing

Contributing Writer, Science, News and Comment, Research News (1990-95).
Science Writer (AAAS mass media fellowship), Detroit Free Press (Summer 1990).

Honors

• University Scholar, UIUC, 2006-2007 (5 or 6 selected from campus every year).
• The International Raymond and Beverly Sackler Prize in Biophysics, Tel Aviv University, 2006.
• Faculty Member of the Precision Proteomics Research Theme, Institute for Genomic Biology, 2005-
• Fellow, American Physical Society, 2004-
• Physics John Bardeen Sony Faculty Scholar: Aug. 04-Aug 07.
• Michael & Kate Bárány Award for Young Investigators, Biophysical Society, 2004.
• Biophysical Society Council, 2004.
• Received National Science Foundation CAREER Award--a most prestigious honor to "support exceptionally promising college and university junior faculty who are committed to the integration of research and education" (quote from the NSF), 2000.
• Named Cottrell Scholar, Research Corporation (fewer than twenty of these awards are made annually), 2000.
• Xerox Award for Faculty Research (one of three Asst. Professors in the College of Engineering) Univ. of Illinois, 2000.
• Beckman Fellow, Center for Advanced Studies, University of Illinois at Urbana-Champaign, Spring 2000.
• Listed in Offices of Instructional Resources "Lists of Teachers Ranked as Excellent," for P140,   Fall 1999, Fall 2001, for Phys 398bio, Spr 2002, Spr 2003.
• Research Innovation Award, Research Corp. 1999.
• Dye development work featured in News section of Analytical Chemistry.
• 1999 Fluorescence Young Investigator Award, Biophysical Society.
• Muscle and mysosin research featured in Biophotonics International magazine, May 1999, June 2006.
• Principal Investigator of NIH R01 grant ranked in top 9.8% (2006), 10.4% (2005), 4.4% (2003), 0.2% (2000) and 2.4% (1996)
• Co-Author of NIH grant ranked in top: 4% (4-yr funding, 1994); 11% (5-yr funding, 1988).
• Searle Foundation nominee from Univ. of Illinois, 1997.
• Invited Speaker at over 100 universities and conferences 1996-present.
• AAAS Mass Media Science and Engineering Fellow, 1990.
• Office of Technology Assessment Congressional Fellow Winner, 1990.
• National Science Foundation Graduate Student Fellowship, 1983-86.
• University of Michigan: Honors Program, 1979-82.
• Member: Amer. Assoc. Adv. of Science; American Physical Society; Biophysical Soc.

Publications

    Papers in Process:

1. Erdal Toprak, Ahmet Yildiz, Steven S. Rosenfeld, and Paul R. Selvin. Why Kinesin Is Remarkably Processive, Submitted PNAS, August 28, 2008.
2. Jeff G Reifenberger, Erdal Toprak, Dan Safer, H. Lee Sweeney, and Paul R. Selvin. Myosin VI undergoes a 180° Power stroke implying an uncoupling of the front lever arm. Submitted, Nature Structural and Molecular Biology, September 23, 2008.
3. Sheyum Syed, Fiona E. Muellner, Paul R. Selvin, Fred J. Sigworth. Hidden Markov models for molecular motors: Application to in
   vitro and in vivo data. Submitted, Nature Methods, June 16, 2008.
4. Comert Kural, Michael Nonet, Paul R. Selvin. FIONA on the living worm, C. elegans. Submitted, Biophysical Journal, July 17, 2008.

Published Technical Papers:

1. Igor M. Kulic, Andre E.X. Brown, Hwajin Kim, Comert Kural, Benjamin Blehm, Paul R. Selvin, Philip C. Nelson and Vladimir I. Gelfand. The Role of Microtubule Movement in Bidirectional Organelle Transport. PNAS,105, 10011–10016 (July 22, 2008). Supporting information.
2. David J. Posson and Paul R. Selvin. The Shaker K+ Channel S4 Voltage Sensor Undergoes a 10 Å Translation during Gating as Measured by Luminescence Energy Transfer Experiments, Submitted Neuron, Feb. 8, 2008; resubmitted April 6, 2008; accepted May 1, 2008. Neuron, Vol 59, 98-109, 10 July 2008. Supplemental Data. General news: www.nigms.nih.gov: Ion Channels Caught in Opening Act, July 9, 2008, University of Illinois at Urbana-Champaign
3. Pinghua Ge and Paul R. Selvin. New Nine- or Ten-dentate Luminescent Lanthanide Chelates. Bioconjugate Chem. 19(5), 1105-1111 (2008). Epub 2008 Apr 29.
4. Hasan Yardimci, Marilyn van Duffelen, Yinghui Mao, Steven S. Rosenfeld, Paul R. Selvin. The mitotic kinesin CENP-E is a processive transport motor. PNAS 105 (16): 6016–6021, April 22, 2008. General news: http://www.news.uiuc.edu/news/08/0513proteins.html
5. Paul R. Selvin: In vitro & in vivo FIONA and other acronyms for watching molecular motors walk. Single Molecules: A Laboratory Manual. Cold Spring Harbor Press. Edited by Paul R. Selvin, Taekjip Ha, Univ. of Illinois, 2007.
6. Watching Molecular Motors Walk with FIONA, Cold Spring Harbor on-line Protocols, 2007: www.cshblogs.org/cshprotocols/
7. Hyokeun Park, Erdal Toprak, Paul R. Selvin: Single-molecule fluorescence to study molecular motors. Quarterly Review of Biophys. 40(1):87-111, Feb 2007.
8. Erdal Toprak, Hamza Balci, Benjamin H. Blehm, Paul R. Selvin.Three-Dimensional Particle Tracking via Bifocal Imaging. Nano Lett., 7 (7), 2043 -2045, 2007. {commentary on article }
9. H. Lee Sweeney, Hyokeun Park, Alan B. Zong, Paul R. Selvin, and Steven S. Rosenfeld. How Myosin VI Coordinates its Heads During Processive Movement EMBO J. 2007 Jun 6;26(11):2682-92. Epub 2007 May 17.{supplemental figure 1}{supplemental figure 2}{supplemental figure 4}{supplemental figure 5}{supplemental figure 6}{supplemental figure 7}{supplemental figure 9}{supplemental figure 10}
10. Ming Xiao, Matthew P. Gordon, Angie Phong, Connie Ha, Ting-Fung Chan, Dongmei Cai, Paul R. Selvin, Pui-Yan Kwok. Determination of Haplotypes from Single DNA Molecules: A Method for Single Molecule Barcoding. Human Mutation; 28(9):913-21, Sep. 2007. eprint April 18, 2007.
11. Comert Kural, Anna S. Serpinskaya, Ying-Hao Chou, Robert D. Goldman, Vladimir I. Gelfand, and Paul R. Selvin. Tracking melanosomes inside a cell to study molecular motors and their interaction.PNAS 104;5378-5382 (March 2007) (originally published online Mar 16, 2007) {supplemental figure 1}{supplemental figure 2} {supplemental figure 3}
12. Hwajin Kim, Shuo-Chien Ling, Gregory C. Rogers, Comert Kural, Paul R. Selvin, Stephen L. Rogers, and Vladimir I. Gelfand. Microtubule binding by dynactin is required for microtubule organization but not cargo transport. J. Cell Biology 176, 641-651 (Feb 26, 2007).{journal cover}
13. Erdal Toprak, Paul R. Selvin: New Fluorescent Tools for Watching Nanometer-Scale Conformational Changes of Single Molecules, Annual Review of Biophysics and Biomolecular Structure, Vol. 36, pg. 349-69, 2007).
14. Hyokeun Park, Anna Li, Li-Qiong Chen, Anne Houdusse, Paul R. Selvin, and H. Lee Sweeney. The unique insert at the end of the myosin VI motor is the sole determinant of directionality. PNAS 104, 778–783 (January 16, 2007). {movie 1} {movie 2} {movie 3} {movie 4}{commentary on article}
15. Ming Xiao, Angie Phong, Connie Ha, Ting-Fung Chan, Dongmei Cai, Lucinda Leung, Eunice Wan, Amy L. Kistle, Joseph L. DeRisi, Paul R. Selvin, Pui-Yan Kwok. Rapid DNA Mapping by fluorescent single molecule detection. Nucleic Acids Research, 35, e16 (2007){published on line - Nucleic Acids Research, Dec. 14, 2006}. {supporting materials}
16. Jessica Richardson, Rikard Blunck, Pinghua Ge, Paul R. Selvin, Francisco Bezanilla, Diane Papazian, Ana M. Correa: Distance measurements reveal a common topology of prokaryotic voltage-gated ion channels in the lipid bilayer. Proc Natl Acad Sci 103(43):15865-70 (2006 Oct 24). Epub 2006 Oct 16.{Supplemental Figure 6}{Supplemental Figure 7}{Supplemental Figure 8}{Supplemental Figure 9a}{Supplemental Figure 9b}{Supplemental Figure 10}
17. Jörg Enderlein, Erdal Toprak, and Paul R. Selvin: Polarization Effect on Position Accuracy of FIONA. Optics Express. J. 14: 8111-8120 (4 September 2006)
18. Lorin S. Milescu, Ahmet Yildiz, Paul R. Selvin, Frederick Sachs: "Extracting dwell time sequences from processive molecular motor data,(Biophys J, Vol. 91, pg. 3135-3150, November 2006).
19. Lorin S. Milescu, Ahmet Yildiz, Paul R. Selvin, Frederick Sachs: "Maximum likelihood estimation of molecular motor kinetics from staircase dwell time sequences," Biophys J. ;91(4):1156-68 (2006 Aug 15). Epub 2006 May 5.
20. Sheyum Syed, Greg E. Snyder, Clara Franzini-Armstrong, Paul R. Selvin, Yale, E. Goldman: Simultaneous detection of the position and structural dynamics of myosin V. (on-line, April 6) EMBO J. May 3; 25(9):1795-803 (2006){cover}
    
21. Erdal Toprak, Joerg Enderlein, Sheyum Syed, Sean A. McKinney, Rolfe G.Petschek, Taekjip Ha, Yale E. Goldman, Paul R. Selvin. Defocused orientation and position imaging (DOPI) of myosin V. Proc Natl Acad Sci U S A 103, (April 17) 6495-9 (2006). {Biophotonics article}
22. Hyokeun Park, Bugs Ramamurthy, Dan Safer, Mirko Travaglia, Li-Qiong Chen, Clara Franzini-Armstrong, Paul R. Selvin & H. Lee Sweeney. Full-length myosin VI dimerizes and moves processively along actin filaments upon monomer clustering, (Molecular Cell, 21, 331–336, February 3, 2006). {University of Illinois News Bureau online article}{journal cover}{Peckham Commentary from Nature}
23. Comert Kural, Hamza Balci, Paul R. Selvin. Molecular Motors One at a Time: FIONA to the Rescue. Journal of Physics Condensed Matter: Special issue on Molecular Motors, 17, S3979-S3995 (2005).
24. Hamza Balci, Taekjip Ha, H. Lee Sweeney, Paul R. Selvin. Interhead distance in myosin VI via SHRIMP supports a hand-over-hand model with a long lever arm. (eprint Apr 29, 2005; Biophys. J., 89(1): 413-7, July 2005 ).
25. Takeshi Sakamoto, Ahmet Yildiz, Paul R. Selvin, and James R. Sellers. Step-size is Determined by Neck Length in Myosin V. Biochemistry. 2005 Dec 13;44(49):16203-16210.
26. David J. Posson, Pinghua Ge, Christopher Miller, Francisco Bezanilla & Paul R. Selvin. Small vertical movement of a K+ channel voltage-sensor measured with luminescence energy transfer. Nature, 436, 848-851(2005).
27. Paul R. Selvin: Walk like a molecular motor, The Scientist, Vol. 19, June 20, 2005. {journal cover}
28. Jeff G. Reifenberger, Pinghua Ge, Paul R. Selvin: Progress in Lanthanides as Luminescent Probes. Annual Reviews in Fluorescence. Editors: Geddes, C.D., Lakowicz, J.R., Springer (2005).
29. Comert Kural, Hwajin Kim, Sheyum Syed, Gohta Goshima, Vladimir I. Gelfand, Paul R. Selvin. Kinesin & Dynein Move a Peroxisome In Vivo: A Tug-of-War or Coordinated Movement? (PDF) (Science Express, April 7, 2005; Science, 308, 1469-1472(2005)).{supplemental material}{Kural Article}{supplemental material- Nature Reviews}{JCB}{Biophotonics International article, June 2005}
30. Ahmet Yildiz, Paul R.Selvin. Fluorescence Imaging with One Nanometer Accuracy (FIONA): Application to Molecular Motors. Accounts of Chemical Research,38(7), 574-82 (2005)
31. Ahmet Yildiz, Paul R.Selvin. Kinesin: walking, crawling, or sliding along? Trends in Cell Biology, 15, 112-120(2005).
32. Hyokeun Park, George Hanson, Steven Duff, Paul Selvin. Nanometer Localization of Single ReAsH Molecules. Journal of Microscopy, 216, 199-205(2004).
33. Pinghua Ge and Paul R. Selvin. Carbostyril derivatives as antenna molecules for luminescent lanthanide chelates. Bioconjugate, Chem. 2004, 15, 1088-1094 (2004).
34. Gregory E. Snyder, Takeshi Sakamoto, John A. Hammer, III, James R.Sellers, and Paul R. Selvin. Nanometer Localization of Single Green Fluorescent Proteins: Evidence that Myosin V Walks Hand-Over-Hand via Telemark Configuration Biophys. J., 87: 1776-1783 (2004).
35. Ahmet Yildiz, Hyokeun Park, Dan Safer, Zhaohui Yang, Li-Qiong Chen, Paul R.Selvin & H. Lee Sweeney. Myosin VI steps via a hand-over-hand mechanism with its lever arm undergoing fluctuations when attached to actin. J. Biol. Chem., 279: 37223-37226 (2004).{UIUC College of Engineering Link}{journal cover}
36. Matthew P. Gordon, Taekjip Ha, Paul R. Selvin: Single Molecule High Resolution Imaging with Photobleaching, P.N.A.S. 101: 6462-6465 (2004). {general description}
37. Ahmet Yildiz, Michio Tomishige, Ronald D. Vale, Paul R. Selvin. Kinesin Walks Hand-Over-Hand. Science, 303, 676-678 (2004). {UIUC Physics Link}, {supporting online materials}
38. Jeff Reifenberger, Gregory E. Snyder, Gordon A. Baym, Paul R. Selvin: Emission Polarization Properties of Europium and Terbium Chelates. J. Phys. Chem B , 107, 12862-12873 (2003).
    
39. Tania Chakrabarty, Chris Yengo, Corry Baldacchino, Li-Qiong Chen, H. Lee Sweeney, Paul R. Selvin: Does the S2 rod of Myosin II uncoil upon two-headed binding to actin? A zippered HMM study. Biochemistry, 42, 12886-12892 (2003).
40. Pinghua Ge, Paul R. Selvin: Thiol-reactive Lanthanide Chelates, II. Bioconjugate Chem., 14, 870-876 (2003).
41. Ahmet Yildiz, Joseph N. Forkey, Sean A. McKinney, Taekjip Ha, Yale E. Goldman, Paul R. Selvin. Myosin V walks hand-over-hand: Single fluorophore imaging with 1.5 nm localization. Science, 300 (5628) 2061-2065 (online June 5, 2003; in print June 27, 2003). {supporting online materials}, {movie}, {perspective}, {cover illustration}{UIUC Physics link}
    
42. Ming Xiao, Jeffrey G. Reifenberger, Amber L Wells, Li-Qiong Chen, Pinghua Ge, H. Lee Sweeney, Paul R. Selvin: An Actin-dependent conformational change in myosin. Nature Structural Biology, 10, 402-408 (May, 2003).
43. Paul R. Selvin: "Lanthanide-labeled DNA" in Topics in Fluorescence Spectroscopy, Vol 7, 177-212,  Joseph Lakowicz, ed., Kluwer Academic/Plenum, New York (2003).
44. Matthew P. Gordon, Paul R. Selvin: A Microcontroller-Based Failsafe for Single Photon Counting Modules. Rev. Sci. Instruments, 74(2), 1150-1152 (2003). [SOURCE CODE] [Reprinted in Virtual Journal of Biological Physics Research, Feb. 1, 2003, www.vjbio.org.]
    
45. Paul R. Selvin: Lighting up Single Ion Channels, Biophys. J.  "New and Notable" Commentary, 84, 1-2 (2003).
46. Michael D. Purdy, Pinghua Ge, Jiyan Chen,  Paul R. Selvin  and  Michael C. Wiener: Thiol-reactive lanthanide chelates for  phasing of  protein x-ray diffraction data, Acta Crystallographica Section D (58: 1111-1117 (2002).
47. Paul R. Selvin: Principles and Biophysical Applications of Luminescent Lanthanide Probes, Annual Review of Biophysics and Biomolecular Structure. Vol. 31, pp. 275-302, (2002).
48. Tania Chakrabarty, Ming Xiao, Roger Cooke, Paul R. Selvin: Holding two heads together: Stability of the Myosin II rod measured by resonance energy transfer between the heads. Proc. Nat'l. Acad. Sci., USA,  99: 6011-6016 (2002).
49. Ming Xiao and Paul R. Selvin: Quantum yields of luminescent lanthanide chelates and far-red dyes measured by resonance energy transfer. J. Amer. Chem. Soc. 123 (29), 7067-7073 (2001).
50. Paul R. Selvin: The renaissance of fluorescence resonance energy transfer (invited review). Nature Structural Biology 7(9), 730-734 (2000).
51. Jiyan Chen, Paul R. Selvin:  Synthesis of 7-Amino-4-trifluoromethyl-2-(1H)-quinolinone and its Use as an Antenna Molecule for Luminescent Europium Polyaminocarboxylates Chelates. Photochem. Photobio. A: Chemistry, 135, 27-32 (2000). 
52. Jiyan Chen, Paul R. Selvin: Lifetime and color-tailored fluorophores in the micro- to milli-second time regime. J. Amer. Chem. Soc. 122(4) 657-660 (2000).
53. Albert Cha, Gregory E. Snyder, Paul R. Selvin, Francisco Bezanilla: Atomic scale movement of the voltage sensing region in a potassium channel measured via spectroscopy. Nature 402,  809-813 (1999).
54. Ming Xiao, Paul R. Selvin: An Improved Instrument for Measuring Time-resolved Lanthanide Emission and Resonance Energy Transfer. Review of Scientific Instruments 70 (10), 3877-3881 (1999).
55. Elise Burmeister Getz, Ming Xiao, Tania Chakrabarty, Roger Cooke, Paul R. Selvin: A Comparison Between the Sulfhydryl Reductants Tris(2-carboxyethyl)phosphine and Dithiothreitol for use in Protein Biochemistry. Analytical Biochemistry 273 (10) 73-80 (1999).
56. Jiyan Chen, Paul R. Selvin: Thiol-reactive Luminescent Chelates of Terbium and Europium. Bioconjugate Chemistry 10 (2), 311 -315 (1999).
57. Paul R. Selvin: "Luminescent Lanthanide Chelates for Improved Resonance Energy Transfer and Applications to Biology" in Applied Fluorescence in Chemistry, Biology and Medicine, edited by W. Rettig, B. Strehmenl, S. Schrader, Springer Verlag, pp 457-487 (1999).
58. Ming Xiao, Handong Li, Gregory E. Snyder, Roger Cooke, Ralph G. Yount, Paul R. Selvin: Conformational Changes between the Active-site and Regulatory Light Chain of Myosin as Determined by Luminescence Resonance Energy Transfers: The effect of nucleotides and actin. Proc. Nat'l Acad. Sci., USA. 95, 15309-15314 (1998).
59. Elise Burmeister Getz, Roger Cooke, Paul R. Selvin: Luminescence Resonance Energy Transfer Measurements in Myosin. Biophysical J. 74 (5) 2451-2458 (1998).
60. Gyorgy Vereb, Elisabeth Jares-Erijman, Paul R. Selvin, Thomas M. Jovin: Temporally and Spectrally Resolved Imaging Microscopy of Lanthanide Chelates. Biophysical J. 74 (5) 2210-2222 (1998).
61. Taekjip Ha, Thilo Enderle, Daniel S. Chemla, Paul R. Selvin, Shimon Weiss: Quantum Jumps of Single Molecules at Room Temperature. Chem. Phys. Letters 271, 1-5 (1997).
62. Min Li, Paul R. Selvin: Amine-reactive Forms of a Luminescent DTPA Chelate of Terbium and Europium: Attachment to DNA and Energy Transfer Measurements. Bioconjugate Chemistry 8 (2), 127-132 (1997).
63. Paul R. Selvin: Lanthanide-based Resonance Energy Transfer. IEEE J. of Selected Topics in Quantum Electronics: "Lasers in Biology" 2 (4), 1077-1087 (1996).
64. Taekjip Ha, Thilo Enderle, Daniel S. Chemla, Paul R. Selvin, Shimon Weiss: Single Molecule Dynamics Studied by Polarization Modulation. Physical Review Letters 77 (19), 3979-3982 (1996).
65. Taekjip Ha, Thilo Enderle, D. Frank Ogletree, Daniel S. Chemla, Paul R. Selvin, Shimon Weiss: Probing the Interaction between Two Single Molecules: Fluorescence Resonance Energy Transfer between a Single Donor and a Single Acceptor Molecule. Proc. Nat. Acad of Sci. 93 (13), 6264-6268 (1996).
66. Paul R. Selvin, Jaru Jancarik, Min Li, Li-Wei Hung: Crystal Structure and Spectroscopic Characterization of a Luminescent Europium Chelate.   Inorganic Chemistry 35, 700-705 (1996).
67. Min Li, Paul R. Selvin: Luminescent Lanthanide Polyaminocarboxylate Chelates: The Effect of Chelate Structure. J. of the American Chemical Soc. 117, 8132-8138 (1995).
68. Paul R. Selvin: "Fluorescence Resonance Energy Transfer," in Methods in Enzymology (Vol. 246, Biochemical Spectroscopy, ed. Kenneth Sauer) Ch 13, 300-334 (1995).
69. Paul R. Selvin, Tariq, M. Rana, John E. Hearst: Luminescence Resonance Energy Transfer.  J. of the American Chemical Soc., Communication to the Editor, 116, 6029-6030 (1994).
70. Paul R. Selvin, John E. Hearst: Luminescence Energy Transfer Using a Terbium Chelate: Improvements on Fluorescence Energy Transfer.  Proc. Nat. Acad. of Sci. 91, 10024-10028 (1994).
71. Paul R. Selvin, David N. Cook, Ning G. Pon, William R. Bauer, Melvin P. Klein, John E. Hearst: Torsional Rigidity of Positively and Negatively Supercoiled DNA. Science 255, 82-85 (1992).
72. Paul R. Selvin, Bethe A. Scalettar, John P. Langmore, Daniel Axelrod, Melvin P. Klein, John E. Hearst: A Polarized Photobleaching Study of Chromatin Reorientation in Intact Nuclei.  J. of Molecular Biology 214, 911-922 (1990).
73. Bethe A. Scalettar, Paul R. Selvin, Daniel Axelrod, Melvin P. Klein, John E. Hearst: A Polarized Photobleaching Study of DNA Reorientation in Agarose Gels.  Biochemistry 29, 4790-4798 (1990).
74. Bethe A. Scalettar, Paul R. Selvin, Daniel Axelrod, John E. Hearst, Melvin P. Klein: A Fluorescence Photobleaching Study of the Microsecond Reorientation of DNA. Biophysical J. 53, 215-226 (1988).
75. Marshall D. Graham, Paul R. Selvin: Separation of Lanthanide-Binding Cells. IEEE Transactions on Magnetics, Jan. 1983.

+ 12 peer-reviewed Conference Proceedings

1. Paul R. Selvin:  "Semiconductor Luminescence Quenchers for Detecting Proximal Molecular Binding Events," U.S. Patent Application, Serial #09/430,384, Oct. 28, 1999, Allowed Feb 11, 2003.
2. Paul R. Selvin, Matthew Gordon, Pui-Yan Kwok, Ming Xiao, and Ting-Fung Chan: "Polynucleotide Barcoding," U.S. Patent Application, Serial No. 10/976,546, Filed Oct 28, 2004.
3. Paul R. Selvin, Pinghua Ge: "Luminescent Lanthanide Binding Chelates," U.S. (and International) Patent Application, Ref. No. TF05135, Filed Nov. 11, 2005.
   
4. a) Paul R. Selvin, John E. Hearst: Luminescent Lanthanide Chelates and Methods of Use. U.S. Patent numbers 5,622,821 [issued April 22, 1997], 5,639,615 [issued June 17, 1997], 5,656,433 [issued August 12, 1997].)
   b) International patents: Australia (AU Pat No 688,928); Canada (CIPO Pat No 2,193,501); Japan (JPO Pat No. 3814291); European: B94-044EU. (Also filed in: Great Britain, France, Germany, Switzerland, and Sweden.)
5. Paul R. Selvin, Jiyan Chen: "Improved Sensitizers for Luminescent Europium Chelates," U.S. Patent Application, Serial #60/167,144, Nov. 23, 1999.

Magazine Articles for General Science Audience:

1. The Future University: Leaner and Meaner? Science Vol. 270 Oct. 6, 1995 p. 135-136.
2. Harrison Case: No Calm After the Storm. Science Vol. 262 Oct. 15, 1993, p. 324-327.
3. Sex Discrimination: Jenny Harrison Finally Gets Tenure in Math at Berkeley. Science Vol. 261 Jul. 16, 1993, p. 286.
4. How Do Particles Put on Weight? Science Vol 259 Jan. 8 1993, p. 173-174.
5. A Gauntlet of Tests for the Theory. Science Vol 259 Jan. 8 1993, p. 174.
6. Future Conditional: Math Education - Multiplying the Meager Numbers. Science Vol 258, Nov. 13, 1992, p. 1200-1201.
7. AAAS Mass Media Newsletter - June 1992. Guest column.
8. "The Big Speedup" (DNA Sequencing) Science Vol. 257, Aug. 14, 1992, p. 885.
9. "The Dyers Hand" (DNA Detection) Science Vol. 257, Aug. 14, 1992, p. 886.
10. "Genentechies Split on Biodiversity Treaty" Science Vol. 257 July 17, 1992, p. 323.
11. "Profile of a Field: Mathematics - Heroism is Still the Norm" Science Vol. 255 March 13, 1992, p. 1382-1383 (reprinted in Math Education society quarterly).
12. "Does the Harrison Case Reveal Sexism in Math?" Science Vol. 252 June 28, 1991, p. 1781-1783.
13. "Is There a Massive Neutrino?" and "Dreaming Up New Flavors" Science Vol. 251 March 22, 1991, p. 1426-1428.
14. "The Raging Bull of Berkeley" and "Is Vincent Sarich Part of a National Trend?" Science Vol. 251, Jan. 25, 1991, p. 368-371.
15. "Alaskan Pork: Aurora Fantasia" Science Vol 250, Nov. 23, 1990, p. 1073.
16. "Charlie Cantor Gets Kicked Upstairs" Science Vol. 249, Sept. 14, 1990, p. 1238-1239.
17. "Graduate students make history" Progressive, Vol. 53, p19, October 1989.
18. "Computer Science: Campus Hackers and the Pentagon" The Nation, Nov. 28, 1988.

As a Science Writer at the Detroit Free Press while on a AAAS 1990 Fellowship I wrote 18 articles, ranging from cryonics, to tracing evolution via gene sequencing of different organisms, to the search for the original language, to technological advances in energy-efficiency.

Current Support

1. PI, Dynamics of Kinesins, Dyneins, and Myosins. NIH AR44420 (continuation), 8/06-7/11: $2.98M.
   
2. PI, NIH, GM 068625: Nanometric Fluorescence Imaging of Single Motor Proteins. To develop FIONA applied to in vitro kinesin, myosin V, myosin VI. 5/1/04-4/30/09, $2.1M
3. PI, NIH, GM 074770: Fluorescence Changes in Shaker Potassium Ion Channel. To measure conformational changes in Shaker as a function of Voltage. 8/1/05-7/31/09.
   
4. PI, NIH, GM. Genetically Targetable Labels for Light and EM: (Roger Tsien, UCSD, PI). Develop new GFP-like fluorophores. 07/04 - 06/08.

Recent Past Support

1. PI, Advanced Fluorescence Energy Transfer on Actomyosin. NIH AR44420, 8/01-7/06: $1.81M.
2. PI (with Ali Yazdani, S. Lance Cooper), Novel Nanoscale Probes of Single Biomolecules and Biomolecular Assemblies, Dept. of Energy DEFG02-91ER45439 (through the Frederick Seitz Material Research Laboratory): 1/00- 1/05 $60k-$80k/yr.
3. PI (with Taekjip Ha): Instrument Development for Imaging and Manipulation of Single Biomolecules. NSF DBI-02-15869, 06/01/02 - 5/31/05: $500k
   
4. PI, Career: Conformational Changes in Voltage-controlled Ion Channels Measured by Advanced Fluorescence Techniques, NSF MCB99-84841, 01/01/00 - 12/31/05, Total: $500k.
5. PI, Turning on and off voltage-gated ion channels: Is the on-off switch a plunger or rotating knob? Cottrell Scholars Fund, Research Corp. 05/01/00 - 04/30/05: $75,000
6. PI, Watching Individual Ion Channels Move Using Advanced Fluorescence Methods: A Biophysical Study of the Proteins that Enable us to Think, See, Hear, Feel and Move. Funded by the Carver Trust. 10/31/99 - 03/30/03: $325,000.
7. PI, Luminescence Resonance Energy Transfer on Actomyosin. NIH, 09/30/96 - 08/01/01. $525k total.
8. PI, Luminescent Lanthanide Chelate Development, Sponsored Research. Funded by Amersham Life Sciences, Inc. Total : $285,000; Period: 07/01/97 - 06/30/00.
9. PI, The Conformation of Voltage-controlled Ion Channels in Living Cells Studied by Fluorescence and Luminescence Resonance Energy Transfer. Funded by Research Corporation. Total Direct: $35,000; Period: 05/01/99 - 04/30/00.
10. PI, Structure of the Myosin Dimer and Molecular Mechanism of Smooth Muscle Regulation. Funded by UIUC Research Board. Total Direct: $25,200; Period: 05/01/98 - 12/31/99.

Invited Talks

2009

• 2nd Annual Midwest Conference for Undergraduate Women in Physics, UIUC, January 16-18, 2009
• Physics & Astronomy Colloquium, UCLA, Feb 12, 2009.
• "In vitro and in vivo; kinesin and myosin moving one (or a few) at a time." Advanced Single Molecule Fluorescence Techniques in Vitro and in Vivo Workshop, 2009 Biophysical Society Meeting, Boston MA, March 1, 2009.
• Colloquium talk, Physics Department at the University of Wisconsin-Milwaukee, April 10, 2009.
• "In vitro and in vivo motility of molecular motors." 15th International Workshop on Single Molecule Detection and Spectroscopy", Berlin, Germany, September 15 – 18, 2009.

2008

• "Kinesin: Single Molecule Studies in vitro and in vivo". Department of Biochemistry, University of Illinois, Urbana-Champaign, February 22, 2008.
• Novel Approaches to Bio-imaging Seminar, Janelia Farm,Chevy Chase, MD, March 9-11, 2008.
• "FIONA on Kinesin, in vitro and in vivo." Seminar, Emory University, Atlanta, GA, March 25, 2008.
• "Single Molecular Motors in vitro and in vivo." International Conference on Intracellular Transport and Trafficking, Goettingen, Germany, April 3-4, 2008.
• "Molecular Motors One Step at a Time: In Vitro & In Vivo." Biomedical Engineering  Dept. Seminar Series, MIT, Cambridge, MA, April 17, 2008.
• "Single Molecule Studies of Kinesin in vitro and in vivo". Department of Biomedical Engineering, University of UCincinnati , May 19, 2008.
• "Kinesin in vivo: Many motors moving?" Gordon Research Conference, "Power-Strokes and Ratchets, " Colby-Sawyer College, New London, NH, June 29-July 4, 2008.
• 2008 "Single Molecule Approaches to Biology" Gordon Research Conference Colby-Sawyer College, New London, NH, August 17 - 22, 2008.
• "Matter alive – or what can a physicist see in biology." Osher Lifelong Learning Institute, UIUC, Oct 14, 2008.
• "Single Molecule Studies of Kinesin and Dynein in vitro and in vivo". Albert Einstein College of Medicine of Yeshiva University, October 22, 2008.
• "In vitro and in vivo; kinesin and myosin moving one (or a few) at a time." Biochemistry Seminar Series, Department of Biochemistry & Molecular Biophysics, Columbia University, New York, NY, October 23, 2008.
• "International Symposium on Molecular and System Life Sciences", Wako, Japan, Dec 10-11, 2008.
  

2007 (18)

• Condensed Matter & Biological Physics Seminar, Purdue University, West Lafayette, IN, January 12, 2007.
• Single Molecule Biophysics, Aspen, Colorado, February 4-10, 2007.
• Physics Colloquium, McGill University, Montreal, Quebec, Canada, March 23, 2007.
• Seminar, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA, April 12, 2007.
• New Directions in Quantitative Biology Symposium, Harvard, Cambridge, MA, April 21, 2007.
• "Single Molecule Mechanics of Motor Proteins, In vitro and In vivo." Cell Biology and Physiology Seminar, The University of Pittsburgh, May 9, 2007.
• "Jump vs. Rotation: How does the Voltage Sensor in K+ Channels Move?" Department of Structural Biology Seminar, The University of Pittsburgh, May 10, 2007.
• Mechanics of Life: from Biomolecules to Molecular Machines Workshop, University of Michigan, Ann Arbor June 11-13, 2007.
• Molecular Motors, CalTech Nanomechanics Summer School, July 3-7, 2007.
• Life Sciences 2007, SECC, Glasgow, July 8-12, 2007.
• Workshop on Mechano Sensitivity of Cells, University of Illinois at Urbana-Champaign, July 30-Aug 3, 2007.
• American Chemical Society, Single Molecule Spectroscopy, Boston, MA Aug. 19-23, 2007.
• Keynote Speaker, 2007 American Society of Biomechanics Conference, Stanford University, Palo Alto, CA, August 23-25, 2007.
• Biochemistry and Molecular Pharmacology Seminar Series, Univ. of Massachusetts Medical School, Worcester, MA, September 19, 2007.
• JFRC Conference: Fluorescent Proteins & Biological Sensors, Janelia Farm, Howard Hughes Medical Institute, Ashburn, VA, October 28-31, 2007.
• "Single Molecule Mechanics of Motor Proteins, in vitro and in vivo" 96th International Titisee Conference "Trends in Biological Optical Microscopy", Titisee, Germany, November 14-18, 2007
• Keynote Speaker, Symposium on Single Molecule Studies at the 47th Annual Meeting of the American Society for Cell Biology, Washington, D.C., December 1-5, 2007.
• "Single Molecule Mechanics of Motor Proteins, In vitro and In vivo." Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, December 19, 2007.
  

2006 (22)

• Colloquium, Center for Computer Integrated Systems for Microscopy and Manipulation, University of North Carolina, Chapel Hill, NC, March 6, 2006.
  
• Single Molecule Biology, Churchill College, University of Cambridge, March 26-30, 2006.
• "Single Molecule Mechanics of Motor Proteins, In vitro and In vivo." Colloquia on Theoretical and Applied Mechanics, Northwestern University, Evanston, IL, April 14, 2006.
• Seminar, "Single Molecule Studies of Motor Proteins, In vivo." Live Cell Imaging, National Institutes of Health, April 19, 2006.
• Materials Research Society (MRS) Spring 2006 Meeting, San Francisco, CA, April 21, 2006.
• Kavli Institute of Physics, UC-Santa Barbara, April 26, 2006.
• "Single Molecule Measurements of Motor Proteins, In vitro and In vivo." Seminar, Molecular Biophysics, University of California, San Diego, CA, May 11, 2006.
• Seminar, Department of Cell and Developmental Biology at Oregon Health and Science University, Portland, OR, May 15, 2006.
• "Single Molecule Measurements of Molecular Motors, in virto & in vivo." Seminar to accept the International Raymond and Beverly Sackler Prize in Biophysics. Tel Aviv University, Tel Aviv, Israel, May 23, 2006.
• Seminar, Department of Biochemistry, Molecular Biology and Cell Biology at Northwestern University, Evanston, IL, June 5th 2006.
• Gordon Conference on Single Molecule Approaches to Biology, Colby-Sawyer College, New London, NH, June 18-23, 2006.

• "Cytoplasmic Dynein Moves Processively by Alternating Movements of Its Two Motor Domains," Protein Society, San Diego, CA, Aug. 6, 2006 (presented by Ahmet Yildiz).
• "Single Molecule Mechanics of Motor Proteins, In vitro and In vivo," Dept of Mechanical & Industrial Engineering (Bio-Interest Group Seminar Series), Sept. 1, 2006 (presented by Hamza Balci).
  
• "Molecular motor dynamics in vitro and in vivo," International Symposium on Bio-nanosystems, Matsushima, Japan, Sept. 2, 2006 (presented by Sheyum Syed).
  
• Frontiers in Single-Molecule Biophysical Chemistry and Imaging" for the 232nd ACS National meeting, San Francisco, Sept. 11, 2006 (presented by Ahmet Yildiz).
• "Single Molecule Measurements of Molecular Motors, in vitro & in vivo" Seminar, Department of Chemistry,Wayne State University, Detroit, MI, Sept. 20, 2006 (presented by Hamza Balci).

Molecular Motors: Point Counterpoint at the Biophysical Society Discussion meeting, Monterey, California, Oct. 19-21, 2006.

• "Single Molecule Measurements of Molecular Motors, in vitro & in vivo," Colloquium, Dept. of Physics, Illinois State University, Oct. 24, 2006. (to be presented by Hamza Balci)
• Third Annual Omaha Imaging Symposium 2006, Omaha, NE, Oct. 28, 2006. (to be presented by Erdal Toprak)
  

Focus on Imaging 2006 Symposium, Hospital for Sick Children in Toronto, Canada, November 2, 2006.

Cornell Biophysics Colloquium, Department of Neurobiology & Behavior, Cornell University, Nov. 15, 2006.

Annual Grantee Workshop for the Exploratory Centers for High Resolution Imaging Probes: UCSD, La Jolla, CA, Dec 8, 2006.

2005 (24)

• Single Molecule Biophysics, Aspen, Colorado, January 2- 8, 2005.
• "Fluorescence Imaging with One Nanometer Accuracy (FIONA): Application to Molecular Motors."Caltech, Pasedena, CA, Jan. 31, 2005.
  
• VII Annual Linz Winter Workshop, Linz, Austria, February 5-7, 2005.
• "New forms of FIONA applied to Myosin V and VI," Motility Subgroup meeting, Biophysical Society Meeting, Long Beach California, February 12, 2005.
• New fluorescent tools for watching nanometer-scale conformational changes of single molecules.Young Investigator Award Presentation, Biophysical Society meeting, Long Beach, CA, February 15, 2005.
• "Fluorescence Imaging with One Nanometer Accuracy (FIONA): Application to Molecular Motors." Univ. of California, Berkeley Physics Department Colloquium, February 28, 2005.
  
• "Fluorescence Imaging with One Nanometer Accuracy (FIONA): Application to Molecular Motors."University of Pennsylvania Physics Dept. Colloquium, Philadelphia, PA, March 16, 2005.
• "Big Jump vs. Little Jump: How does the Voltage Sensor in K+ Channels Move?" Biophysics Lecture Series, Caltech, Pasadena, CA, April 4, 2005.
  
• "Big Jump vs. Little Jump: How does the Voltage Sensor in K+ Channels Move?" Molecular, Cellular and Integrative Neurosciences Program, Colorado State Univ. April 6, 2005.
• "Fluorescence Imaging with One Nanometer Accuracy (FIONA): Application to Myosin, Kinesin, and Dynein." The 13th annual Bud Suddath Symposium in Biosciences, Georgia Institute of Technology, April 15-16 , 2005.
• Chemistry Seminar, Oberlin College, Oberlin, OH , May 4, 2005.
  
• "Molecular Motors I." and "Molecular Motors II, DNA Haplotyping, and Ion Channels." Institute for Mathematics and its Application, Minneapolis, MN, May 16-20, 2005.
• Nobel Symposium on Controlled Nanoscale Motion in Artificial and Biological Systems, Bäckaskog Castle, Sweden, June 13 - 17, 2005.
• 6th International Weber Symposium on Innovative Fluorescence Methodologies in Biochemistry and Medicine, Kauai, Hawaii, July 22-28, 2005
• Physics Department Colloquium, Univ. of Colorado, Boulder Colorado, Sept. 7, 2005
• 11th International Workshop on "Single Molecule Spectroscopy and Ultra Sensitive Analysis in the Life Sciences," Berlin, Germany, September 21-23, 2005.
• School of Physics, Georgia Institute of Technology, Atlanta, Georgia, October 5, 2005.
  
• Wake Forest University, Winston-Salem, North Carolina, Oct. 6, 2005.
• RICE Physics and Astronomy Department Colloquium, Houston, TX, Oct. 19, 2005.
  
• "Nanobiology: Studying Single-Molecule Molecular Motors." Department of Chemistry and Biochemistry,University of California, Los Angeles, November 15, 2005.
• Seminar, Department of Molecular and Integrative Physiology, Univ. of Illinois, Urbana-Champaign, December 1, 2005.
• "Exploratory Centers for the Development of High Resolution Probes for Cellular Imaging," NIH, Dec 5-6, 2005.
  
• Optical Probes in Cellular and Molecular Imaging /Beyond the Visible: Optical Probe Design and Applications for Living Cells. Sonoma Valley, CA, December 8-9, 2005
• "Myosin VI: monomer or dimer?" American Society for Cell Biology, Mini-symposium on Motility, San Francisco, CA, December 11, 2005.
  

2004 (6)

• "How Kinesin Moves," Motility Subgroup, Biophysical Society meeting, Baltimore, February 15, 2004, (presented by Ahmet Yildiz).
  

• "How molecular motors move", Understanding Complex Systems, Univ. of Illinois, Urbana-Champaign, May 18, 2004.
  
• Biophysical Chemistry and Novel Imaging of Single Molecules and Simple Cells, Symposium at the 228th American Chemistry Society Meeting, Philadelphia, August 22-26, 2004.
• Yale University, Department of Cellular and Molecular Physiology, September 16, 2004.
• Univ. of Penn., Dept. of Biochemistry and Biophysics, Oct 20, 2004
• University of Illinois, Chicago Physics Dept. Colloquim, Chicago, IL, Nov. 3, 2004.
  

2003 (14)

• Aspen Single Molecule Biophysics Conference, Aspen, CO, January 7-10, 2003.
• Nanometer resolution with single molecule fluorescence imaging: Application to biomolecular motors. Dept. of Physics, Case Western Reserve University, Jan 22, 2003.
• UIUC Physics Dept. Colloquium, Urbana, IL, February 6, 2003.
• Cornell Physics Dept. Colloquium, Ithaca, NY, February 10, 2003.
• New & Notable Symposium, "Fluorescence Imaging with One Nanometer Accuracy: Application to Myosin V." 47th Annual Meeting of the Biophysical Society, San Antonio, TX,  March 2003.
• Nanometer resolution with single molecule fluorescence imaging: Application to biomolecular motors., Univ. of Michigan Physics Dept. Colloquim, Ann Arbor MI, April 9, 2003 (cancelled).
• Single fluorophore imaging with 1.5 nm accuracy shows Myosin V walks hand-over-hand. Cardiovascular Research Institute, UCSF, April 18, 2003.
• Harvard Physical Chemistry seminar, Boston, MA, May 1, 2003.
• Single molecule fluorescence imaging at the nanometer-scale: Application to biomolecular motors. Univ. of Oregon, Dept. of Physics Colloquim, June 5, 2003.
• Single fluorophore imaging with 1.5 nm accuracy: Application to biomolecular motors. Physical Chemistry seminar, UCLA, Sept 29, 2003.
• 1.5 nanometer localization of single fluorophores: Application to biomolecular motors. US-Swiss 2003 Forum on Nanoscale Science and Nanotechnology, University of Basel, Switzerland, Oct 13-15, 2003.
• Lanthanides and other new luminescent probes for high throughput screening. Roche Pharmaceuticals, Basel, Switzerland, Oct 15, 2003.
• Single fluorophore imaging with 1.5 nm accuracy: Application to biomolecular motors. Physics Dept. Colloquim, Ecole Normale Supérieure de Paris, France, Oct 16, 2003.
• 1.5 nanometer localization of single fluorophores: Application to biomolecular motors. Center for Theoretical Biophysics, UCSD, Nov. 21, 2003.
  

2002 (5)

• Nanometer-resolution studies of molecular motors and ion channels.
  Understanding Complex Systems Symposium, May 15, 2002, Urbana IL.
• High resolution fluorescence studies of molecular motors and ion channels,  Department of Chemistry and Biochemistry, University of Delaware, Sept 16, 2002.
• Nanometer-scale Fluorescence Measurements of Molecular Motors (& Ion Channels), Univ. of Rochester, Dept. of Biochemistry and Biophysics, Oct 16, 2002.
• How molecular motors move: a single molecule fluorescence study. Department of Chemistry and Biochemistry, University of California, UCSD, Oct 24, 2002.
• Two nm resolution with single molecule fluorescence imaging: Application to molecular motors.  Imaging & Optics Seminar, Department of Pharmacology, Univ. Nevada, Reno, Nov 14, 2002.

2001 (8)

• "Conformational changes in voltage-controlled ion channels (nerves) and actomyosin (muscle), detected with new fluorescence techniques," University of Illinois at Chicago, Dept. of Pharmacology, March 9, 2001.
• "Lanthanides as unusual fluorescent probes," Univ. of Maryland, March 23, 2001.
• ICAS 2001: New fluorescent probes and detection systems in bioscience, Waseda University, Japan (declined).
• "Advances in Optics for Biotechnology, Medicine and Surgery: Novel Optical Sensing Technologies," Banaff, Canada, July 22-27, 2001 (declined).
• "The Role of Actin and the S2 Rod in Myosin Conformation and Motility," Society of General Physiologists National Meeting, Woods Hole, MA, September 6-8, 2001.
• "Conformational Changes in Actomyosin and Ion Channels Measured by Fluorescence," MIT, Biological Chemistry, November 5, 2001.
• "Conformational Changes in Actomyosin and Ion Channels Measured by Fluorescence," Brandeis University, Chemistry Dept., November 6, 2001.
• "Nanometer-scale shape changes in actomyosin (muscle) and ion-channels (nerves) measured by new fluorescence techniques." Scuola Normale Superiore, Pisa Italy, November 16, 2001. (canceled)

2000 (10)

• "Conformational changes in actomyosin (muscle) and voltage-controlled ion channels (nerves) detected with new fluorescence techniques," Univ. of Washington, Seattle, January 19, 2000.
• "How nerves fire," Department of Physics, UIUC, February 25, 2000.
• "Conformational changes in actomyosin (muscle) and voltage-controlled ion channels (nerves) detected with new fluorescence techniques," Florida State University, Tallahassee, FL,  February 29, 2000.
• "Conformational changes in ion channels (and actomyosin) measured with new fluorescence techniques," Univ. of Michigan, Dept of Physics and Biophysics, Res. Div., March 31, 2000.
• "In search of new luminescent probes: the ion channel benchmark," NIH, April 17-18, 2000.
• "Conformational changes in actomyosin and ion channels measured with advanced fluorescence techniques," NIH, April 19, 2000.
• Panelist, "Biological nanostructures," Nanoscience and Nanotechnology Symposium, NIH, June 25-26, 2000.
• "Conformational changes in actomyosin (muscle) and voltage-controlled ion channels (nerves) detected with new fluorescence techniques," University of Chicago, Oct 19, 2000.
• "How nerves fire," November 4, 2000, University of Illinois Saturday Honors Program.
• "Conformational changes in actomyosin (muscle) and voltage-controlled ion channels (nerves) detected with new fluorescence techniques." Univ. of Texas UT Southwestern Medical Center, November 9, 2000.

1999 (13)

• Young Fluorescence Investigator Award Presentation, Biophysical Society Meeting, Baltimore, MD, February 1999.
• "Sub-nanometer resolution with optical photons:  Application to Muscles and Nerves," University of Illinois at Urbana-Champaign, Physics Colloquium, February 25, 1999.
• "Sub-nanometer resolution with optical photons: Detection of conformational changes in actomyosin (muscle) and voltage-controlled ion channels (nerves)," University of Virginia, Charlottesville, VA, April 29, 1999.
• Conformational changes in actomyosin (muscle) and voltage-controlled ion channels (nerves) detected with new fluorescence techniques. St. Louis University,  May 12, 1999.
• "Time-resolved fluorescence and novel energy transfer techniques for sensitive detection of biomolecular interactions: potential applications in high-throughput screening." Sigma Chemical Co., St. Louis, MO, May 21, 1999.
• "Single molecule detection," International meeting of Istituto Nazionale Fisica della Materia, Sicily, June, 1999.
• "Muscle and Contractility."  Invited Poster, Gordon Conference, New London, MJ, June 6-9, 1999.
• "Advanced Resonance Energy Transfer techniques: Application to Muscle and Nerves," 4th International Weber Symposium on Innovative Fluorescence Methodologies in Biochemistry and Medicine, Maui, Hawaii, June 23-27, 1999.
• "Single-molecule and Ensemble measurements in Ion Channel," International conference on Single Molecule Biophysics, Louve, France, July, 1999.
• "Luminescent lanthanides: their photophysics and use in resonance energy transfer on biological systems,"  Federation of Analytical Chemistry and Spectroscopy Societies, Vancouver, Canada, October 24-29, 1999.
• "Application of fluorescence in biotechnology and drug research," The Royal Danish School of Pharmacy, Copenhagen, Denmark, October 29, 1999.
• "Conformational changes in ion channels (nerves) and actomyosin (muscle) measured by advanced fluorescence methods,"  Univ. of Illinois, Theoretical Biophysics seminar, November 29, 1999.
• "Measuring conformational changes in actomyosin and voltage-controlled ion channels using new forms of fluorescence energy transfer," University of Pennsylvania, December 6, 1999.

1998 (7)

• Biophysics Society Meeting, Kansas City, MO, February 22-26, 1998.
• "Lanthanides: A probe for many seasons", Advances in Molecular Labels, Signaling and Detection conference, San Diego, May 4-5, 1998.
• Department of Molecular Biophysics and Physiology, seminar speaker, Rush Medical Center, October 2, 1998.
• Condensed Matter Seminar, University of Illinois - Chicago, October 16, 1998.
• "Advances in Resonance Energy transfer: Application to actomyosin and ion channels," Cell and Molecular Biology Training program Symposium: University of Illinois, Urbana, October 31, 1998.
• 2nd International Assay Development for High-Throughput Screening Conference and Cell Lines Symposium. January 14, 1998 (invited but declined).
• Eastern Analytical Symposium, November 15-20, 1998 in Somerset, NJ (invited but declined).

1997 (5)

• Biophysics Society, New Orleans, LA, February 1997.
• 3rd International Conference on Methods and Applications of Fluorescence, Maui, Hawaii, June 1997.
• 5th International Conference on Methods and Applications of Fluorescence, Spectra, Berlin Germany, September 21-24, 1997.
• Boston Biomedical Research Inst., Harvard Med. School, Boston MA, December 10, 1997.
• New England Nuclear Corp. , Boston MA, December 11, 1997.

1996 (7)

• University of California, San Diego (UCSD), San Diego, CA, 1996.
• University of Illinois (UIUC), Urbana, IL, 1996.
• University of New Mexico, Albuquerque, NM, 1996.
• Caltech, Pasadena, CA, 1996.
• Rutgers, Piscataway, NJ, 1996.
• Wake Forest, Winston, NC, 1996.
• Biophysical Society, 1996.

Updated:  09/29/2008